Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation-Reference-Cited by-同舟云学术

Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation

Published:2018 Issue:4 Volume:67 Page:535-543
ISSN:1341-1357
Container-title:Experimental Animals
language:en
Short-container-title:Exp. Anim.

Author:

Nakagawa Yoshiko¹,Sakuma Tetsushi²,Takeo Toru¹,Nakagata Naomi¹,Yamamoto Takashi²

Affiliation:

1. Center for Animal Resources and Development (CARD), Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan

2. Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan

Publisher

Japanese Association for Laboratory Animal Science

Subject

General Veterinary,General Biochemistry, Genetics and Molecular Biology,Animal Science and Zoology,General Medicine

Link

https://www.jstage.jst.go.jp/article/expanim/67/4/67_18-0062/_pdf

Reference31 articles.

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2. 2. Chen S., Lee B., Lee A.Y., Modzelewski A.J., and He L. 2016. Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes. J. Biol. Chem. 291: 14457–14467.

3. 3. Cradick T.J., Qiu P., Lee C.M., Fine E.J., and Bao G. 2014. COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites. Mol. Ther. Nucleic Acids 3: e214.

4. 4. Hashimoto M. and Takemoto T. 2015. Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Sci. Rep. 5: 11315.

5. 5. Hashimoto M., Yamashita Y., and Takemoto T. 2016. Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Dev. Biol. 418: 1–9.

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