Sevoflurane suppresses hepatocellular carcinoma cell progression via circ_0001649/miR-19a-3p/SGTB axis
Nai Sun1, Jianzhuang Gong2, Wei Zhang1, Xiaochen Yang1 and Jiaying Liu1
1Department of Anesthesiology and 2Department of Gastroenterology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou City, Henan Province, China
Corresponding Author: Jiaying Liu, Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 16 Tongbai North Road, Zhengzhou City, 450007 Henan Province, China. e-mail: ljy185062@163.com
Summary. Background. Sevoflurane is a widely used anesthetic agent and is reported to play an anti-tumor action in many cancers. However, the underlying mechanisms are largely unclear.
Methods. Hepatocellular carcinoma (HCC) cells were treated with sevoflurane for 12 or 24 h. HCC cell proliferation, migration, invasion, and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay, transwell assay, and flow cytometry assay, respectively. The protein levels were determined by western blot. The expression of circular RNA (circ)_0001649, microRNA (miR)-19a-3p, and small glutamine rich tetratricopeptide repeat containing Beta (SGTB) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between miR-19a-3p and circ_0001649 or SGTB was predicted by Starbase and confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays.
Results. Sevoflurane inhibited HCC cell proliferation, migration, and invasion, but promoted apoptosis. Sevoflurane could affect the expression of circ_0001649 and knockdown of circ_0001649 reversed the effects of sevoflurane on HCC cell progression. Subsequently, miR-19a-3p was identified as a target of circ_0001649 and directly targeted SGTB. In addition, circ_0001649 suppressed the development of sevoflurane-induced HCC cells through miR-19a-3p/SGTB axis.
Conclusion. Our study demonstrated that sevoflurane inhibited HCC cell development via circ_0001649/miR-19a-3p/SGTB axis. Histol Histopathol 38, 537-547 (2023)
Key words: Sevoflurane, Hepatocellular carcinoma, circ_0001649, miR-19a-3p, SGTB
DOI: 10.14670/HH-18-484
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ŠThe Author(s) 2023. Open Access. This article is licensed under a Creative Commons CC-BY International License. |
