Hypomethylation-mediated overexpression of ITGA2 stimulates cell invasion and migration of thyroid carcinoma
Hong Chen, Chunying Zhang, Yanbing Li and Chunyou Chen
Department of Head and Neck Surgery, Tangshan Gongren Hospital, Tangshan, China
Corresponding Author: Chunyou Chen, Department of Head and Neck Surgery, Tangshan Gongren Hospital, No.27 Wenhua Road, Lubei District, Tangshan, 063000, China. e-mail: chunyouccchen@163.com
Summary. Objective. To study the molecular mechanism of DNA methylation-mediated ITGA2 overexpression in thyroid carcinoma (TC).
Methods. First, 450K methylation data and mRNA expression profiles in TCGA-THCA dataset were downloaded from TCGA database. ITGA2 was identified as a methylation-driven gene by using R package “MethylMix”. Afterwards, qRT-PCR, western blot and flow cytometry assay were performed to measure ITGA2 expression in TC cells. Methylation-specific PCR was utilized to measure promoter region methylation of ITGA2 in TC cells. Transwell and wound healing assays were carried out to assess cell invasive and migratory properties.
Results. Compared with normal cells, TC cells presented significantly increased ITGA2 expression. In addition, ITGA2 expression was controlled by DNA methylation. Hypomethylation of CpG island resulted in an increased ITGA2 expression. Hence, methylation and expression levels of ITGA2 were inversely associated. Moreover, overexpression of ITGA2 and promoter region hypomethylation facilitated cell invasive and migratory abilities in TC.
Conclusion. These findings authenticated that promoter region hypomethylation of ITGA2 fostered ITGA2 expression as well as TC cell invasion and migration. Histol Histopathol 38, 787-796 (2023)
Key words: ITGA2, Promoter methylation, Invasion and migration, Thyroid carcinoma
DOI: 10.14670/HH-18-552
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©The Author(s) 2023. Open Access. This article is licensed under a Creative Commons CC-BY International License. |
