EVALUATION AND COMPREHENSIVE COMPARISON OF OCTADECYLAMINE AND STEARAMIDE-BASED SOLID LIPID NANOPARTICLES FOR EFFICIENT GENE DELIVERY
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Published:2023
Issue:1
Volume:14
Page:1-15
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ISSN:2572-4258
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Container-title:Nanoscience and Technology: An International Journal
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language:en
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Short-container-title:Nano Sci Technol Int J
Author:
Kirmizitaş Fatma Ceren,Varlı Hanife Sevgi,Türkoglu Nelisa
Abstract
In recent years various nonviral vector systems have been developed to overcome the disadvantages of viral vectors. Thus, researchers have turned to more secure and easy to reach vector systems. In this study, we have evaluated the gene transfer efficiency of two different nonviral vectors prepared using two lipids: octadecylamine and stearamide. The solid lipid nanoparticles were produced with the emulsion-solvent evaporation method. Particles' mean size, polydispersity index (PDI), and zeta potential values for octadecylamine nanoparticle (ONP) are, respectively, 382.7 nm (± 20), 0.4, and 28 mV (±
2.03) and for stearamide nanoparticle (SNP) are 276.9 nm (± 10.45), 0.3, and 20.4 mV (± 4.03), as determined by using Zetasizer via the dynamic light scattering (DLS) method. Morphological characterization of nanoparticles was performed with scanning electron microscope (SEM) and atomic force microscope (AFM). The chemical characterization was performed by Fourier-transform infrared spectroscopy (FTIR). The cytotoxicity of nanoparticles were determined via 3-(4,5-Dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay with an MCF-7 human breast cancer cell line. The cytotoxicity for both ONP and SNP were calculated over 55%. Also nanoparticles/plasmid DNA conjugates cytotoxicity were determined with no significant toxicity obtained. The transfection efficiency was evaluated by observing green fluorescent protein (GFP) expression in the MCF-7 human breast cancer cell line. The transfection efficiency was 67.5% (± 2.3) for ONP, 77.6% (± 1.2) for SNP, and 70.7% (± 1.4) for commercially available transfection agent.
Subject
Mechanics of Materials,Condensed Matter Physics,General Materials Science
Reference40 articles.
1. Alkan, F., Varli, H.S., Demirbilek, M., Kaplan, E., and Lafin, N.T., A Cationic Stearamide-Based Solid Lipid Nanoparticle for Delivering Yamanaka Factors: Evaluation of the Transfection Efficiency, Chem. Open, vol. 9, no. 11, pp. 1181-1189, 2020. 2. Allen, C., Qiu, T.A., Pramanik, S., Buchman, J.T., Krause, M.O., and Murphy, C.J., Research Highlights: Investigating the Role of Nanoparticle Surface Charge in Nano-Bio Interactions, Environ. Sci.: Nano, vol. 4, no. 4, pp. 741-746, 2017. 3. Almofti, M.R., Harashima, H., Shinohara, Y., Almofti, A., Baba, Y., and Kiwada, H., Cationic Liposome-Mediated Gene Delivery: Biophysical Study and Mechanism of Internalization, Arch. Biochem. Biophys, vol. 410, no. 2, pp. 246-253, 2003a. 4. Almofti, M.R., Harashima, H., Shinohara, Y., Almofti, A., Li, W., and Kiwada, H., Lipoplex Size Determines Lipofection Efficiency with or without Serum, Mol. Membrane Biol., vol. 20, no. 1, pp. 35-43, 2003b. 5. Benitez, J.J., San-Miguel, M.A., Dominguez-Meister, S., Heredia-Guerrero, J.A., and Salmeron, M., Structure and Chemical State of Octadecylamine Self-Assembled Monolayers on Mica, J. Phys. Chem. C, vol. 115, no. 40, pp. 19716-19723, 2011.
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