Detection of Methicillin-resistant Staphylococcus aureus (MRSA) resistant to vancomycin and linezolid in bulk tank milk by E-test method

Author:

ONARAN ACAR Bahar1ORCID,KEYVAN Erhan2ORCID

Affiliation:

1. ANKARA ÜNİVERSİTESİ VETERİNER FAKÜLTESİ

2. MEHMET AKIF ERSOY UNIVERSITY

Abstract

Staphylococcus aureus is considered a serious threat to public health, besides is one of the most common causes of subclinical mastitis in dairy cows. Therefore, especially methicillin-resistant Staphylococcus aureus (MRSA) is among the most critical reasons for antibiotic treatment. Monitoring the antibiotic resistance of MRSA from livestock animals and foods is of great significance. The E-test method is a suitable option for detecting antibiotics' minimum inhibition concentration (MIC) value because it is an easy and fast assay to perform. This study aimed to detect vancomycin, teicoplanin, and linezolid resistance of milk-borne 34 MRSA isolates with subclinical mastitis by the E-test method to determine MIC values. The study determined that 8.8% of MRSA isolates isolated from milk with subclinical mastitis were also resistant to vancomycin and 11.7% to linezolid, while none of the isolates were determined to be resistant to teicoplanin, according to EUCAST. The data obtained from the study reveal that the efficacy of vancomycin and linezolid antibiotics, which are commonly used against MRSA infections, are in danger. Especially before MRSA treatment, MIC values of antibiotics should be determined, and appropriate antibiotics should be used in effective doses.

Publisher

Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi

Reference26 articles.

1. 1. Adegoke, A. A., & Okoh, A. I. (2014). Species diversity and antibiotic resistance properties of Staphylococcus of farm animal origin in Nkonkobe Municipality, South Africa. Folia Microbiologica, 59, 133-140. https://doi.org/10.1007/s12223-013-0275-1

2. 3. Al-Amery, K., Elhariri, M., Elsayed, A., El-Moghazy, G., Elhelw, R., El-Mahallawy, H., El Hariri, M., & Hamza, D. (2019). Vancomycin-resistant Staphylococcus aureus isolated from camel meat and slaughterhouse workers in Egypt. Antimicrobial Resistance & Infection Control, 8, 1-8. https://doi.org/10.1186/s13756-019-0585-4 3.5. Aires-de-Sousa, M. (2017). Methicillin-resistant Staphylococcus aureus among animals: current overview. Clinical Microbiology and Infection, 23(6), 373-380. https://doi.org/10.1016/j.cmi.2016.11.002

3. 6. Algammal, A. M., Enany, M. E., El-Tarabili, R. M., Ghobashy, M. O., & Helmy, Y. A. (2020). Prevalence, antimicrobial resistance profiles, virulence and enterotoxins-determinant genes of MRSA isolated from subclinical bovine mastitis in Egypt. Pathogens, 9(5), 362. https://doi.org/10.3390/pathogens9050362

4. 8. Bouzidi, S., Bourabah, A., Cheriet, S., Abbassi, M. S., Meliani, S., & Bouzidi, H. (2023). Occurrence of virulence genes and methicillin-resistance in Staphylococcus aureus isolates causing subclinical bovine mastitis in Tiaret area, Algeria. Letters in Applied Microbiology. ovad003, https://doi.org/10.1093/lambio/ovad003 10. Campos, B., Pickering, A. C., Rocha, L. S., Aguilar, A. P., Fabres-Klein, M. H., de Oliveira Mendes, T. A., Fitzgerald, J. R. & & de Oliveira Barros Ribon, A. (2022). Diversity and pathogenesis of Staphylococcus aureus from bovine mastitis: current understanding and future perspectives. BMC Veterinary Research, 18(1), 1-16. https://doi.org/10.1186/s12917-022-03197-5

5. 12. Centers for Disease Control and Prevention (2019). 2019 AR Threats Report. https://www.cdc.gov/drugresistance/biggest-threats.html 14. Charlton, C. L., Hindler, J. A., Turnidge, J., & Humphries, R. M. (2014). Precision of vancomycin and daptomycin MICs for methicillin-resistant Staphylococcus aureus and effect of subculture and storage. Journal of Clinical Microbiology, 52(11), 3898-3905. https://doi.org/10.1128/JCM.01571-14

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