Assessment of Viral load for Human Cytomegalovirus in Patients with Hepatitis B Virus

Abstract

      This study was carried out on Seventy-Six samples, formed (55 males and 21 females) with age ranging (11-72) year were collected from center Health Laboratory/Al-Hakeem Hospital, and AL-Sadder medical city in AL-Najaf city, during the period from January (2013) to August (2013). Fifteen healthy individuals without any evidence of chronic inflammatory disease involved as control, age ranging (21-50) years. All patients were divided in six age groups. Blood samples were collected from patients and control for immunological (IgM, IgG & TFN-α) by using ELISA and molecular study by RT-PCR, respectively. The results showed  that 76 HBsAg seropositive in all age groups but the age group (44-54) year revealed high significant(p<0.05) than other age groups and male more infection than female. The ELISA test results showed that 68 (89.4%) out of the 76 samples were positive for anti-HCMV IgG antibodies, and 4(5.26%) samples were positive for anti-HCMV IgM. The results of the Real-Time PCR revealed that HCMV DNA were detected in 23(30.2%) out of 76 patients were found in all age groups with viral loads ranging from (0.24- 1730000) Copies/ml, and the results of controls group in Real-Time PCR were HCMV negative. The results of cytokines profile (TNF-α) showed a highly significant (P<0.05) elevation in the serum of all patients than control groups.