Abstract
Rapid and accurate detection of hydrogen peroxide (H2O2), an important reactive oxygen species (ROS) released from living cells, is of great significance for early diagnosis of tumors. Here, a high sensitive enzyme-free electrochemical sensor for the detection of H2O2 released from living cells was constructed based on MXene@ZIF-8@Pt NPs nanocomposites. Through the characterization of physical and chemical properties, it was observed that Pt NPs with excellent catalytic activity were uniformly supported on MXene@ZIF-8, which exhibited excellent conductivity and large specific surface area. Thanks to the significantly enhanced catalytic activity derived from the successful integration of MXene, ZIF-8 and Pt NPs, under the optimal conditions, the sensing platform based on MXene@ZIF-8@Pt NPs exhibited a wide linear range from 355.4 nM to 21.75 mM, with a limit of detection as low as 120.9 nM, while showing satisfactory reproducibility and selectivity. Furthermore, the developed electrochemical sensor enables real-time monitoring of H2O2 released from living Hela cells under N-formylmethionyl-leucyl-phenylalanine stimulation. Overall, the MXene@ZIF-8@Pt NPs developed in this article will become a promising candidate in monitoring physiological processes.
Funder
National Natural Science Foundation of China
Young Scientists Fund of the National Natural Science Foundation of China
Key Scientific Research Project of Shaanxi Provincial Department of Education
Shaanxi Provincial Natural Science Basic Research Program-Joint Funds of Department of Science and Technology of Shaanxi Province and Shaanxi Coal and Chemical Industry Group Co., Ltd.
Engineering Research Center of Low-Carbon Energy Efficient Utilization, Universities of Shaanxi Province
Young Scientists Project of Shaanxi Province High-Level Talent-Introduction Plan
Youth Project of Natural Science Foundation of Shaanxi Province
Publisher
The Electrochemical Society