Author:
Hsu You-Ren,Lee Geng-Yen,Chyi Jen-Inn,Chang Chung-ke,Huang Chih-Cheng,Hsu Chen-Pin,Huang Tai-huang,Ren Fan,Wang Yu-Lin
Abstract
AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotope-labeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.
Publisher
The Electrochemical Society
Cited by
12 articles.
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