CD155 is essential for skeletal muscle regeneration by regulating satellite cell proliferation and differentiation

Author:

Li Wenpeng1,Zhang Yuan2ORCID,Liu Yitian1,Feng Chongyang1,Duan Chujun2,Zhang Zhixiang1,Zhao Peng1,Zhuang Ran2ORCID,Ding Yong13

Affiliation:

1. Orthopedic Department of Tangdu Hospital Fourth Military Medical University Xi'an Shaanxi China

2. Department of Immunology Fourth Military Medical University Xi'an Shaanxi China

3. Honghui Hospital, Xi'an Jiaotong University Xi'an China

Abstract

AbstractCD155, a member of the immunoglobulin superfamily, is closely related to cell proliferation, adhesion, and migration. CD155 is overexpressed on the surface of cancer cells to promote cell proliferation and is upregulated in damaged tissues as a stress‐induced molecule. The process of skeletal muscle regeneration after injury is complex and involves injurious stimulation and subsequent satellite cell proliferation. However, the role of CD155 in this process remains unelucidated. This study aimed to explore the role of CD155 in injured skeletal muscle regeneration and to clarify its effect on satellite cell proliferation and differentiation. Here, quantitative real‐time polymerase chain reaction (RT‐qPCR) and immunofluorescence results indicated that CD155 expression in satellite cells increased after skeletal muscle injury. CD155 knockout in mice impaired the regeneration of skeletal muscle. A bone marrow transplantation mouse model was constructed and revealed that CD155 on skeletal muscle tissues, not immune cells, affected muscle regeneration. In vitro, CD155 knockdown in myoblasts inhibited their proliferation and differentiation. The transcriptomic analysis also indicated that CD155 absence can impair the biological proliferation and differentiation process of myoblasts. Our research demonstrates that CD155 directly promotes injured muscle regeneration by regulating satellite cell proliferation and differentiation, which may be a potential therapeutic molecule for skeletal muscle injury.

Funder

National Natural Science Foundation of China

Fourth Military Medical University

Publisher

Wiley

Subject

Genetics,Molecular Biology,Biochemistry,Biotechnology

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