Affiliation:
1. Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Women and Children's Hospital of Chongqing Medical University Chongqing China
2. Chongqing Clinical Research Center for Reproductive Medicine Chongqing Health Center for Women and Children Chongqing China
3. College of Life Science Northeast Agricultural University Harbin China
4. College of Animal Science and Technology Jilin Agricultural University Changchun China
5. Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury Third Military Medical University Chongqing China
Abstract
AbstractPostovulatory aging can trigger deterioration of oocyte quality and subsequent embryonic development, and thus reduce the success rates of assisted reproductive technology (ART). The molecular mechanisms underlying postovulatory aging, and preventative strategies, remain to be explored. The near‐infrared fluorophore IR‐61, a novel heptamethine cyanine dye, has the potential for mitochondrial targeting and cell protection. In this study, we found that IR‐61 accumulated in oocyte mitochondria and reduced the postovulatory aging‐induced decline in mitochondrial function, including mitochondrial distribution, membrane potential, mtDNA number, ATP levels, and mitochondrial ultrastructure. In addition, IR‐61 rescued postovulatory aging‐caused oocyte fragmentation, defects in spindle structure, and embryonic developmental potential. RNA sequencing analysis indicated that the postovulatory aging‐induced oxidative stress pathway might be inhibited by IR‐61. We then confirmed that IR‐61 decreased the levels of reactive oxygen species and MitoSOX, and increased GSH content in aged oocytes. Collectively, the results indicate that IR‐61 may prevent postovulatory aging by rescuing oocyte quality, promoting successful rate in ART procedure.
Funder
National Natural Science Foundation of China
Subject
Genetics,Molecular Biology,Biochemistry,Biotechnology