A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance

Author:

Patchsung Maturada1,Homchan Aimorn12,Aphicho Kanokpol1,Suraritdechachai Surased1,Wanitchanon Thanyapat13,Pattama Archiraya4,Sappakhaw Khomkrit1,Meesawat Piyachat1,Wongsatit Thanakrit1,Athipanyasilp Artittaya14,Jantarug Krittapas1,Athipanyasilp Niracha4,Buahom Juthamas4,Visanpattanasin Supapat2ORCID,Niljianskul Nootaree5,Chaiyen Pimchai1,Tinikul Ruchanok2,Wichukchinda Nuanjun3,Mahasirimongkol Surakameth3,Sirijatuphat Rujipas6,Angkasekwinai Nasikarn6,Crone Michael A.789ORCID,Freemont Paul S.789,Joung Julia1011121314,Ladha Alim1011121314,Abudayyeh Omar12ORCID,Gootenberg Jonathan12,Zhang Feng1011121314ORCID,Chewapreecha Claire1516,Chanarat Sittinan2,Horthongkham Navin4,Pakotiprapha Danaya2,Uttamapinant Chayasith1ORCID

Affiliation:

1. School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand.

2. Department of Biochemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand.

3. Division of Genomic Medicine and Innovation Support, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

4. Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

5. PTT Public Company Limited, Bangkok, Thailand.

6. Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

7. London Biofoundry, Imperial College Translation and Innovation Hub, London, United Kingdom.

8. Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, London, United Kingdom.

9. UK Dementia Research Institute Centre for Care Research and Technology, Imperial College London, London, United Kingdom.

10. Howard Hughes Medical Institute, Cambridge, Massachusetts, USA.

11. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

12. McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts, USA.

13. Department of Biological Engineering, MIT, Cambridge, Massachusetts, USA.

14. Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts, USA.

15. Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

16. Wellcome Sanger Institute, Hinxton, United Kingdom.

Publisher

Mary Ann Liebert Inc

Subject

Genetics,Biotechnology

Reference47 articles.

1. Global disparities in SARS-CoV-2 genomic surveillance

2. 3. European Centre for Disease Prevention and Control and Europe WHORO for. Methods for the Detection and Characterisation of SARS-CoV-2 Variants—First Update What Is New in This Update: Key Messages. World Health Organization. Regional Office for Europe: Copenhagen, Denmark; 2021.

3. Lifting of COVID-19 restrictions in the UK and the Delta variant

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