Responses of Saccharomyces cerevisiae Cells Grown in Cultures Prepared from Different Tea Infusions to Oxidative Stress

Author:

KIRMIZIKAYA Görkem1ORCID,OKUTAN Tuba1ORCID,KİREÇCİ Oğuz Ayhan2ORCID,YILMAZ Prof. Dr. Ökkeş1ORCID

Affiliation:

1. FIRAT ÜNİVERSİTESİ, FEN FAKÜLTESİ

2. BİTLİS EREN ÜNİVERSİTESİ, HİZAN MESLEK YÜKSEKOKULU

Abstract

Tea is one of the most consumed beverages. Saccharomyces cerevisiae, a model organism in studies on oxidative stress and toxicology, was used to investigate the effects of tea on oxidative stress induced by H2O2. S. cerevisiae cultures were prepared from black, green and white tea infusions and incubated at 30°C for 72 hours. Glutathione-S-transferase enzyme activity and total protein spectrophotometric, malondialdehyde, glutathione and alpha-tocopherol and ergosterol analyses from cell pellets obtained from cultures were performed by HPLC, and fatty acids were performed by GC device. Although protein level in tea infusion groups was higher (p

Publisher

KSU Journal of Agriculture and Nature

Subject

General Earth and Planetary Sciences,General Engineering,General Environmental Science

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