Evaluation of the Histopathological Changes Accompanied for the Toxic Effects of Diazinon on the Spleen of Mice

Abstract

This study was carried out to determine the histopathological changes caused by diazinon in the spleen of Swiss albino mice. Experimental groups containing low dose (30 mg/kg), medium dose (60 mg/kg) and high dose (120 mg/kg) were exposed to diazinon through oral administration for 30 consecutive days. Separation and hemorrhage in the capsule, congestion, enlarged white pulp, amyloid formation, and karyolysis in some megakaryocytes were determined in the splenic parenchyma of the low dose group. An increase in the number of enlarged white pulps, hemorrhage within splenic parenchyma, accumulation of cells into dilated sinusoids and amyloid formation were examined in the medium dose group. Some cells passing from the splenic parenchyma into dilated sinusoids were also observed. Intensive congestion, necrotic areas within spleen tissue, an increase in the number of karyolytic megakaryocytes, fibrosis and some cells passing from the splenic parenchyma into enlarged sinusoids were prominent histological lesions in the high dose group. These results showed that diazinon caused severe dose-related histopathological damages and had the capacity to disrupt functions of the spleen.