Synergistic effect of Rosemary and Lemon extractions on some physiological and biochemical parameters of CCl4-Stressed male rats

Abstract

This study was carried out to examine the preventive impact of marine extracts of rosemary and lemon individually or together in adult-male rats with liver injury induced by carbon tetrachloride. The extracts were prepared and tested on 40 male rats distributed into groups by collecting blood samples and conducting some hematological and biochemical parameters. CCl4-induced significant increases in the weight of the liver and heart, while rosemary and lemon extracts alone were not able to restore the liver weight, but the effect was in the synergy. As well, the extracts alone or in combination had a significant impact on reducing heart weight. Additionally, CCl4 caused a substantial reduction in RBC, Hb, PCV, MCV, MCHC and lymphocytes, whereas WBC, monocyte and granulocyte increased. While extracts exhibited an enhancement in these parameters, the best effect was when the two extracts were used together. The biochemical parameters indicated high significance in glucose, AST, ALT and triglycerides; however, total protein, albumin, cholesterol and T-AOC decreased compared to the control group. Both rosemary and lemon worked to restore and remove the oxidative effect. Keywords: Rosemary(A plant that grows in mountainous regions), Lemon, Carbon tetrachloride, Stress, Rats.