Isolation, characterization and identification of Klebsiella sp. dor_27f isolated from textile effluents

Abstract

This research study aimed to isolate and characterize a new bacterial strain from textile effluents. To do this, bacteria were cultured using the MSM medium, where a colony was isolated through six successive pickings. It then underwent a DNA extraction process using the phenol-chloroform-isoamyl alcohol methodology, and an electrophoresis was carried out to confirm the extraction. In addition, the isolated colony was identified as Klebsiella sp. by sequencing the 16S rRNA gene using bioinformatics tools. To observe its biotechnological potential, the bacterial strain was grown in an MSM broth enriched with Golden Yellow K2R azoic dye at a concentration of 50 mg/L, showing a percentage of decolorization of 74 % after 72 hours at 37 °C, indicating the potential of the isolated colony for the development of bio-remediation processes for effluents containing azoic dyes. Keywords: Bacterial strain, Klebsiella sp., DNA extraction, decolorization, effluents.