Preparation Protein Hydrolysates from Fish by-product and Study Effected on Lettuce (Lactuca sativa L.) Growth, Yield, Quality and Enhanced Salt Tolerance

Abstract

The experiment was carried out during the winter season 2019-2018 in Al-Hartha district, Basrah Governorate to study the effects of fish by-product prepared by Alcalase and Flavourzyme enzyme as a foliar application on lettuce (Lactuca sativa L.) yield and quality grown under salinity conditions. Fish by-product protein hydrolysate was spray-applied (3 and 6ml. L-1) additional to control treatment (spraying with water) referred to (T0-T4) at four (S4), six(S6) times after 20 days transplanting at10-day intervals. Randomized Complete Block Design (R.C.B.D.) was used as factorial experiment. Treatment means were compared by using Least Significant Differences (L.S.D.) at a probability of 0.05. The results showed that spraying with protein hydrolysates had a significant effect on most studied characters compared to control treatment. Results showed that foliar application with (T2) significantly increased the total leaves number, leaves area, carbohydrate ,proline, shoot fresh weight of plant, dry matter, total soluble solids (TSS %) and total yield additional to significantly decreased in nitrate contents, while foliar application with (T4) significantly increased in the plant height and stem height. The results showed that spraying six times (S6) were significant increases in these growth characters comparing with four sprays (S4). The interaction between treatments and spraying number show significantly increased in some characters, the (T2 S6) had the highest value shoot fresh weight 762.5g and total yield 17.899 tone.donum-1.