An Approach for the Development of Immunochip for the Detection of Celiac Disease based on Anti-Gliadin Antibodies Quantification

Abstract

A simple and rapid nanocomposite-based immunosensor is proposed for diagnosing celiac disease. The disease is highly prevalent, and the average prevalence of the disease has been reported to be between 0.5 and 1% worldwide. The advantage of the developed method is that it is more sensitive and specific for detecting anti-gliadin antibodies elicited in response to gluten ingestion in celiac susceptible individuals. The antigen (gliadin) was immobilized onto the nanocomposite electrode, and subsequently, specific antibodies from the human serum samples were electrochemically detected using 50mM of K3Fe (CN)6. Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) studies were carried out to record the electrochemical response. The modifications at each fabrication step were checked using Field emission-Scanning Electron Microscopy (FE-SEM). The sensor was specific and showed minimal response to non-specific serum proteins. The sensitivity and lower limit of detection of the developed sensor were 762.6µA cm-2ng-1 and 0.2pg per 6µl, respectively.