Affiliation:
1. AMES-Bioengineering, University of California, San Diego, La Jolla 92093.
Abstract
Recent evidence shows that circulating granulocytes play an important role in capillary stasis and tissue injury. We investigated two aspects of the problem in a Wiggers hemorrhagic shock model of the rat: the survival rate and the microvascular no-reflow phenomenon. A conventional group of rats with normal blood cells and a neutropenic group of rats pretreated with intraperitoneal antigranulocyte antibody were used to evaluate the effects of granulocytes. Two hemorrhagic shock protocols (HSP) were carried out. In HSP-1, the rats were subjected to 40 mm Hg mean arterial pressure for 3 hours. The conventional group (n = 11) showed a 36% survival rate compared with 100% in the neutropenic group (n = 6). In HSP-2, the hypotension was more severe, 30 mm Hg mean arterial pressure for 7 hours. There were no survivors in the conventional group (n = 8), compared with a 100% survival rate in the neutropenic group (n = 6). The extent, location, and mechanism of the no-reflow phenomenon was investigated by examining histological sections from several organs after infusion of a contrast medium to mark vessels with flow in a control group without shock and in the HSP-2 model 2 hours after blood replacement. The arterioles and venules uniformly contained contrast medium in all three groups; only capillaries showed no-reflow. A significantly higher percentage of no-reflow was observed in the capillaries of the conventional shock group than in the neutropenic shock group. We concluded that the obstruction of capillaries was largely due to trapped granulocytes, suggesting that these leukocytes play a key role in the capillary no-reflow phenomenon and survival from hemorrhagic shock.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine,Physiology
Reference28 articles.
1. The interplay of central and peripheral factors in irreversible hemorrhagic shock
2. Microvascular adjustments during irreversible hemorrhagic shock in rat skeletal muscle
3. Deformation of white blood cells in capillaries;Bagge U;Adv Microcirc (Karger-Basel),1977
4. Vascular endothelium-leukocyte interaction sticking shear force in venules;Schmid-Schonbein GW;Circ Res,1975
5. MacGregor RR: Granulocyte adherence in Glynn LE Houck JC Weissmann G (eds): The Cell Biology of Inflammation. Handbook of Inflammation vol 2. Amsterdam Elsevier North-Holland Biomedical Press 1980 pp 267-298
Cited by
200 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献