Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells

Author:

Planat-Bénard V.1,Menard C.1,André M.1,Puceat M.1,Perez A.1,Garcia-Verdugo J.-M.1,Pénicaud L.1,Casteilla L.1

Affiliation:

1. From the UMR 5018 UPS CNRS (V.P.-B., M.A., L.P., L.C.), Research Institute 31, France; CRBS CNRS FRE2593 (C.M., M.P.), Montpellier, France; Clinica Universitaria (A.P.), University of Navarra, Pamplona, Spain and Instituto Cavanilles (J.-M.G.), University of Valencia, Valencia, Spain.

Abstract

Cardiomyocyte regeneration is limited in adult life. Thus, the identification of a putative source of cardiomyocyte progenitors is of great interest to provide a usable model in vitro and new perspective in regenerative therapy. As adipose tissues were recently demonstrated to contain pluripotent stem cells, the emergence of cardiomyocyte phenotype from adipose-derived cells was investigated. We demonstrated that rare beating cells with cardiomyocyte features could be identified after culture of adipose stroma cells without addition of 5-azacytidine. The cardiomyocyte phenotype was first identified by morphological observation, confirmed with expression of specific cardiac markers, immunocytochemistry staining, and ultrastructural analysis, revealing the presence of ventricle- and atrial-like cells. Electrophysiological studies performed on early culture revealed a pacemaker activity of the cells. Finally, functional studies showed that adrenergic agonist stimulated the beating rate whereas cholinergic agonist decreased it. Taken together, this study demonstrated that functional cardiomyocyte-like cells could be directly obtained from adipose tissue. According to the large amount of this tissue in adult mammal, it could represent a useful source of cardiomyocyte progenitors.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Cardiology and Cardiovascular Medicine,Physiology

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