Affiliation:
1. From ReNeuron Ltd (T.V., R.P.S., W.P.W., H.H.); Department of Psychology, Institute of Psychiatry, King’s College London (H.H.); and Department of Neurosurgery, Addenbrookes Hospital, Cambridge (J.S.B.), UK.
Abstract
Background and Purpose
—Grafts of MHP36 cells have previously been shown to reduce dysfunction after global ischemia in rats. To test their efficacy after focal ischemia, MHP36 cells were grafted 2 to 3 weeks after transient intraluminal middle cerebral artery occlusion (tMCAO) in rats.
Methods
—MHP36 cells were implanted into the hemisphere contralateral to the lesion, with 8 deposits of 3 μL of cell suspension (25 000 cells per microliter). Sham grafted rats received equivalent volumes of vehicle. Three groups, sham-operated controls (n=11), MCAO+sham grafts (n=10), and MCAO+MHP36 grafts (n=11), were compared in 3 behavioral tests.
Results
—In the bilateral asymmetry test, MCAO+MHP36 grafted rats exhibited neglect before grafting but subsequently showed no significant dysfunction, whereas MCAO+sham grafted rats showed stable sensorimotor deficits over 18 weeks relative to controls. MCAO+sham grafted rats demonstrated spontaneous motor asymmetry and increased rotational bias after injection of dopamine agonists. MCAO+MHP36 and control groups exhibited no bias in either spontaneous or drug-induced rotation. In contrast to motor recovery, MCAO+MHP36 grafted rats showed no improvement relative to MCAO+sham grafted rats in spatial learning and memory in the water maze. MCAO produced large striatal and cortical cavitations in both occluded groups. Lesion volume was significantly reduced (
P
<0.05) in the MCAO+MHP36 grafted group. The majority of MHP36 cells were identified within the intact grafted hemisphere. However, MHP36 cells were also seen in the cortex, striatum, and corpus callosum of the lesioned hemisphere.
Conclusions
—MHP36 cells may improve functional outcome after MCAO by assisting spontaneous reorganization in both the damaged and intact hemispheres.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Advanced and Specialized Nursing,Cardiology and Cardiovascular Medicine,Neurology (clinical)
Reference29 articles.
1. Wolf PA Kannel WB D’Agostino RB. Epidemiology of stroke. In: Ginsberg MD Bogousslavsky J eds. Cerebrovascular Disease. Vol 2. Malden Mass: Blackwell Science; 1998:834–849.
2. Pathobiology of ischaemic stroke: an integrated view
3. Schallert T Kozlowski DA. Brain damage and plasticity: use-related enhanced neural growth and over-use-related exaggeration of injury. In: Ginsberg MD Bogousslavsky J eds. Cerebrovascular Disease. Vol 1. Malden Mass: Blackwell Science; 1998:611–619.
4. Recovery after stroke
Cited by
189 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献