Association of Chromatin Proteins High Mobility Group Box (HMGB) 1 and HMGB2 with Mitotic Chromosomes
-
Published:2003-08
Issue:8
Volume:14
Page:3414-3426
-
ISSN:1059-1524
-
Container-title:Molecular Biology of the Cell
-
language:en
-
Short-container-title:MBoC
Author:
Pallier Coralie12, Scaffidi Paola3, Chopineau-Proust Stéphanie1, Agresti Alessandra3, Nordmann Patrice1, Bianchi Marco E.3, Marechal Vincent2
Affiliation:
1. Unité de Virologie, Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, 94275 Le Kremlin Bicêtre, France 2. Université Pierre et Marie Curie, 75252 Paris, France 3. San Raffaele Scientific Institute, 2032 Milan, Italy
Abstract
High mobility group box (HMGB) 1 and 2 are two abundant nonhistone nuclear proteins that have been found in association with chromatin. Previous studies based on immunofluorescence analysis indicated that HMGB1 dissociates from chromosomes during mitosis. In the present work, HMGB1 and 2 subcellular localization was reinvestigated in living cells by using enhanced green fluorescent protein- and Discosome sp. red fluorescent protein-tagged proteins. Contrary to previous reports, HMGB1 and 2 were shown to be present under two forms in mitotic cells, i.e., free and associated with the condensed chromatin, which rapidly exchange. A detailed analysis of HMGB2 interaction with mitotic chromosomes indicated that two sites encompassing HMG-box A and B are responsible for binding. Importantly, this interaction was rapidly inactivated when cells were permeabilized or exposed to chemical fixatives that are widely used in immunodetection techniques. A comparable behavior was also observed for two proteins of the HMG-nucleosome binding (HMGN) group, namely, HMGN1 and HMGN2.
Publisher
American Society for Cell Biology (ASCB)
Subject
Cell Biology,Molecular Biology
Reference57 articles.
1. Aidinis, V., Bonaldi, T., Beltrame, M., Santagata, S., Bianchi, M.E., and Spanopoulou, E. (1999). The RAG1 homeodomain recruits HMG1 and HMG2 to facilitate recombination signal sequence binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2.Mol. Cell. Biol.19, 6532–6542. 2. Baird, G.S., Zacharias, D.A., and Tsien, R.Y. (2000). Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.Proc. Natl. Acad. Sci. USA97, 11984–11989. 3. Bianchi, M.E. (1995). The HMG-box domain. In:DNA-Protein: Structural Interactions, ed. D. Lilley, Oxford: Oxford University Press, 177–200. 4. Bianchi, M.E., and Beltrame, M. (2000). Upwardly mobile proteins. Workshop: the role of HMG proteins in chromatin structure, gene expression and neoplasia.EMBO Rep.1, 109–114. 5. Boonyaratanakornkit, V., Melvin, V., Prendergast, P., Altmann, M., Ronfani, L., Bianchi, M.E., Taraseviciene, L., Nordeen, S.K., Allegretto, E.A., and Edwards, D.P. (1998). High-mobility group chromatin proteins 1 and 2 functionally interact with steroid hormone receptors to enhance their DNA binding in vitro and transcriptional activity in mammalian cells.Mol. Cell. Biol.18, 4471–4487.
Cited by
122 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|