Affiliation:
1. Departments of Molecular & Human Genetics, Biochemistry & Molecular Biology, Molecular Virology and Microbiology, and the Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, Texas, USA
Abstract
ABSTRACT
In this issue of the
Journal of Bacteriology
, N. J. Bonde, E. A. Wood, K. S. Myers, M. Place, J. L. Keck, and M. M. Cox (J Bacteriol 205:e00184-23, 2023,
https//doi.org/10.1128/jb.00184-23
) used an unbiased transposon-sequencing (Tn-seq) screen to identify proteins required for life when cells lose the RecG branched-DNA helicase (synthetic lethality). The proteins’ identities indicate pathways that prevent endogenous DNA damage, pathways that prevent its homology-directed repair (HDR) “strand-exchange” intermediates between sister chromosomes, and pathways that resolve those intermediates. All avoid intermediate pile-up, which blocks chromosome segregation, causing “death-by-recombination.” DNA damage is managed to regulate crucial but potentially lethal HDR.
Funder
National Cancer Institute
HHS | NIH | NIA | National Institute for Aging
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology