Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos-Reference-Cited by-同舟云学术

Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos

Published:2006-10 Issue:10 Volume:13 Page:1166-1169
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Blacksell Stuart D.¹²³⁴⁵,Smythe Lee¹²³⁴⁵,Phetsouvanh Rattanaphone¹²³⁴⁵,Dohnt Michael¹²³⁴⁵,Hartskeerl Rudy¹²³⁴⁵,Symonds Meegan¹²³⁴⁵,Slack Andrew¹²³⁴⁵,Vongsouvath Manivanh¹²³⁴⁵,Davong Viengmone¹²³⁴⁵,Lattana Olay¹²³⁴⁵,Phongmany Simmaly¹²³⁴⁵,Keolouangkot Valy¹²³⁴⁵,White Nicholas J.¹²³⁴⁵,Day Nicholas P. J.¹²³⁴⁵,Newton Paul N.¹²³⁴⁵

Affiliation:

1. Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic

2. Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, United Kingdom

3. Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok 10400, Thailand

4. WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Coopers Plains, Queensland, 4108, Australia

5. WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, KIT Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands

Abstract

ABSTRACT The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira “gold standard” microscopic agglutination test.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00219-06

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