Considerations for the Analysis of Bacterial Membrane Vesicles: Methods of Vesicle Production and Quantification Can Influence Biological and Experimental Outcomes

Author:

Bitto Natalie J.12ORCID,Zavan Lauren12ORCID,Johnston Ella L.12ORCID,Stinear Timothy P.3ORCID,Hill Andrew F.24ORCID,Kaparakis-Liaskos Maria12ORCID

Affiliation:

1. Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne, Victoria, Australia

2. Research Centre for Extracellular Vesicles, School of Molecular Sciences, La Trobe University, Melbourne, Victoria, Australia

3. Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Parkville, Victoria, Australia

4. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

Abstract

Recent years have seen a surge in interest in the roles of BMVs in host-microbe interactions and interbacterial communication. As a result of such rapid growth in the field, there is a lack of uniformity in BMV enumeration.

Funder

Australian Research Council

La Trobe University

Veski

Department of Health | National Health and Medical Research Council

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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