Affiliation:
1. Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6
Abstract
ABSTRACT
Stenotrophomonas maltophilia
is an emerging nosocomial pathogen that displays high-level intrinsic resistance to a variety of structurally unrelated antimicrobial agents. Efflux mechanisms are known to contribute to acquired multidrug resistance in this organism, and indeed, one such multidrug efflux system, SmeDEF, was recently identified. Still, the importance of SmeDEF to intrinsic antibiotic resistance in
S. maltophilia
had not yet been determined. Reverse transcription-PCR confirmed expression of the
smeDEF
genes in wild-type
S. maltophilia
, and deletion of
smeE
or
smeF
in wild-type strains rendered the mutants hypersusceptible to several antimicrobials, suggesting that SmeDEF contributes to intrinsic antimicrobial resistance in this organism. Expression of
smeDEF
was also enhanced in an in vitro-selected multidrug-resistant mutant, although deletion of
smeF
but not of
smeE
in these mutants compromised antimicrobial resistance. Apparently, hyperexpressed SmeF is capable of functioning with additional multidrug efflux components to promote multidrug resistance in
S. maltophilia
.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
112 articles.
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