The Ovine Cathelicidin SMAP29 Kills Ovine Respiratory Pathogens In Vitro and in an Ovine Model of Pulmonary Infection-Reference-Cited by-同舟云学术

The Ovine Cathelicidin SMAP29 Kills Ovine Respiratory Pathogens In Vitro and in an Ovine Model of Pulmonary Infection

Published:2001-01 Issue:1 Volume:45 Page:331-334
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Brogden K. A.¹,Kalfa V. C.¹,Ackermann M. R.²,Palmquist D. E.³,McCray P. B.⁴,Tack B. F.⁵

Affiliation:

1. Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, Iowa 500101;

2. Department of Veterinary Pathology, Iowa State University, Ames, Iowa 500112;

3. Midwest Area, USDA Agricultural Research Service, Peoria, Illinois 616043; and

4. Departments of Pediatrics4 and

5. Microbiology,5 College of Medicine, The University of Iowa, Iowa City, Iowa 52242

Abstract

ABSTRACT Cathelicidins are antimicrobial peptides from sheep (SMAP29 and SMAP34), rabbits (CAP11 and CAP18), rodents (CRAMP), and humans (FALL39, LL37, and h/CAP18). In a broth microdilution assay against nine ovine pathogens, SMAP29, SMAP34, mouse CRAMP, CAP18, CAP18 31 , CAP18 28 , CAP18 22 , and CAP18 21a were the most active, with MICs as low as 0.6 μg/ml. Other cathelicidins were less active. In lambs with pneumonia, 0.5 mg of SMAP29 reduced the concentration of bacteria in both bronchoalveolar lavage fluid and consolidated pulmonary tissues. Hence, the antimicrobial activity of SMAP29 suggests that it has applications in the treatment of respiratory tract infections.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.45.1.331-334.2001

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