Transcriptional Control of Spliced and Unspliced Human T-Cell Leukemia Virus Type 1 bZIP Factor ( HBZ ) Gene

Abstract

ABSTRACT The human T-cell leukemia virus type 1 (HTLV-1) basic leucine zipper factor ( HBZ ) gene is encoded by the minus strand of the HTLV-1 provirus and transcribed from the 3′ long terminal repeat (LTR). HBZ gene expression not only inhibits the Tax-mediated activation of viral gene transcription through the 5′ LTR but also promotes the proliferation of infected cells. However, the HBZ promoter region and the transcriptional regulation of the gene have not been studied. In this study, we characterize the promoters of the spliced version of the HBZ gene (s HBZ ) and the unspliced version of the HBZ gene (us HBZ ) by luciferase assay. Both promoters were TATA-less and contained initiators and downstream promoter elements. Detailed studies of the promoter for the s HBZ gene showed that Sp1 sites were critical for its activity. The activities of the s HBZ and us HBZ gene promoters were upregulated by Tax through Tax-responsible elements in the 3′ LTR. We compared the functions of the proteins derived from the s HBZ and us HBZ transcripts. sHBZ showed a stronger suppression of Tax-mediated transcriptional activation through the 5′ LTR than did usHBZ; the level of suppression correlated with the level of protein produced. The expression of s HBZ had a growth-promoting function in a T-cell line, while us HBZ expression did not. This study demonstrates that Sp1 is critical for s HBZ transcription, which accounts for the constitutive expression of the s HBZ gene. Functional differences between s HBZ and us HBZ suggest that the s HBZ gene plays a significant role in the proliferation of infected cells.