Metaproteomic Analysis of Gut Resistome in the Cecal Microbiota of Fattening Pigs Raised without Antibiotics

Author:

Buthasane Pamornya1,Roytrakul Sittiruk2,Phaonakrop Narumon2,Tunsagool Paiboon3,Buthasane Wannapol1,Am-in Nutthee4,Suriyaphol Gunnaporn1ORCID

Affiliation:

1. Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

2. Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand

3. Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand

4. Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

Abstract

In order to control and reduce antibiotic use in animals, the Department of Livestock Development, Thailand, has launched a campaign for antibiotic-free livestock production. The present study has shown for the first time that CRISPR-associated endonucleases Cas1 and Cas9, which function to defend against viruses, were markedly observed in Ruminococcus and Lactobacillus , respectively, in ceca of pigs raised without antibiotics (ABF).

Funder

the Agricultural Research Development Agency

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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