Development of a Novel Chikungunya Virus-Like Replicon Particle for Rapid Quantification and Screening of Neutralizing Antibodies and Antivirals-Reference-Cited by-全球学者库

Development of a Novel Chikungunya Virus-Like Replicon Particle for Rapid Quantification and Screening of Neutralizing Antibodies and Antivirals

Published:2023-04-13 Issue:2 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Lin Hui-Chung¹²,Chiao Der-Jiang²,Shu Pei-Yun³,Lin Hui-Tsu²,Hsiung Chia-Chu²,Lin Chang-Chi²⁴,Kuo Szu-Cheng²⁴^ORCID

Affiliation:

1. Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan

2. Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan

3. Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei, Taiwan

4. Department and Graduate Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan

Abstract

This study proposes a transfection-free system that enables the safe packaging of CHIK VRPs with all necessary components via baculovirus transduction. Those mosquito cell-derived CHIK VRP (mos-CHIK VRPs) were shown to recapitulate viral replication and subgenomic dual reporter (enhanced green fluorescent protein [eGFP] and luciferase) expression in infected host cells.

Funder

National Health Research Institutes

Ministry of Science and Technology, Taiwan

MOD | Medical Affairs Bureau

National Defense Medical Center

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.04854-22

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