Bru SIC: a novel selective medium for the primary isolation of Brucella in veterinary samples

Author:

Mena-Bueno Sara12ORCID,Poveda-Urkixo Irati1,Asensio Daniel3,Echarte Iñaki1,Zabalza-Baranguá Ana1,Grilló María Jesús1ORCID

Affiliation:

1. Instituto de Agrobiotecnología (CSIC-Gobierno de Navarra), Mutilva Baja, Spain

2. Universidad Pública de Navarra, Mutilva Baja, Spain

3. Reactivos para Diagnóstico, S.L. Josep Tura, Sentmenat, Spain

Abstract

Farrell’s medium (FM) and CITA medium (CM), recommended for Brucella isolation in animal samples, are inhibitory for certain strains. A reformulated Brucella selective medium (BSM), containing half the CM vancomycin and colistin concentrations, improved the isolation of B. melitensis , but not Brucella abortus bv1.

Funder

Dirección General de Industria, Energia y Proyectos Estrategicos S3, Gobierno de Navarra

MEC | Agencia Estatal de Investigación

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Reference57 articles.

1. Office International of Epizooties, World Organization for Animal Health. 2018. Chapter 3.1.4: brucellosis (Brucella abortus, B. melitensis, and B. suis) (infection with B. abortus, B. melitensis, and B. suis). In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 8th ed. World Organization for Animal Health, Paris, France.

2. A study of the nature of brucellosis in chickens;Felsenfeld O;Am J Vet Res,1951

3. A SELECTIVE MEDIUM FOR THE ISOLATION OF BRUCELLAE FROM CONTAMINATED MATERIALS

4. A selective medium for isolation of Brucella melitensis;Renoux G;Ann Inst Pasteur (Paris),1954

5. A selective medium for the isolation of Brucella abortus from herd samples of milk;Mair NS;Mon Bull Minist Health Public Health Lab Serv,1955

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