High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of Tuberculosis-Reference-Cited by-同舟云学术

High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of Tuberculosis

Published:2022-02-23 Issue:1 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Musisi Emmanuel¹²³,Sessolo Abdul²,Kaswabuli Sylvia²,Zawedde Josephine²,Byanyima Patrick²,Kasinga Shariifah²,Sanyu Ingvar²,Uwimaana Esther⁴,Walimbwa Stanley⁵,Olore Joseph⁵,Ssengooba Willy⁶,Sekaggya Christine⁷,Joloba Moses L.⁸,Worodria William²,Huang Laurence²⁹¹⁰,Gillespie Stephen H.¹,Sloan Derek J.¹,Sabiiti Wilber¹^ORCID

Affiliation:

1. Division of Infection and Global Health, School of Medicine, University of St Andrews, Scotland, United Kingdom

2. Infectious Diseases Research Collaboration, Kampala, Uganda

3. Department of Biochemistry and Sports Sciences, Makerere University, Kampala, Uganda

4. Makerere University Lung Institute, Makerere University, Kampala, Uganda

5. Naguru Referral Hospital, Kampala, Uganda

6. Department of Medical Microbiology, Makerere University, Kampala, Uganda

7. Infectious Diseases Institute, Makerere University, Kampala, Uganda

8. Department of Immunology and Molecular Biology, Makerere University, Kampala, Uganda

9. Division of Pulmonary and Critical Care Medicine, University of California San Francisco, San Francisco, California, USA

10. HIV/AIDS Division, University of California San Francisco, San Francisco, California, USA

Abstract

This paper highlights the value of stool as a sample type for diagnosis of tuberculosis. While other studies have used DNA-based assays like the Xpert MTB/RIF and culture to detect Mycobacterium tuberculosis in stool, this is the first study that has applied TB-MBLA, an RNA-based assay, to quantify TB bacteria in stool.

Funder

European and Developing Countries Clinical Trials Partnership

HHS | National Institutes of Health

Global Challenges Research Fund

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02100-21

Reference54 articles.

1. WHO. 2021. Global tuberculosis report 2021. World Health Organization Geneva Switzerland.