Peripheral Blood Microbiome Analysis via Noninvasive Prenatal Testing Reveals the Complexity of Circulating Microbial Cell-Free DNA

Author:

Tong Xunliang1,Yu Xiaowei2,Du Yang3,Su Fei4,Liu Ye4,Li Hexin4,Liu Yunshan3,Mu Kai5,Liu Qingsong6,Li Hui7,Zhu Jiansheng8,Xu Hongtao9,Xiao Fei410ORCID,Li Yanming1

Affiliation:

1. Department of Pulmonary and Critical Care Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China

2. Center for Reproductive Medicine, Center for Prenatal Diagnosis, First Hospital, Jilin University, Changchun, Jilin, China

3. Annoroad Gene Technology Co., Ltd., Beijing, China

4. Clinical Biobank, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China

5. Department of Medical Genetics, Zibo Women and Children Hospital, Zibo, China

6. Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China

7. Clinical Laboratory, Maternal and Child Health Hospital of Hubei Province, Wuhan, China

8. Medical Genetic Center, Maternity and Child Health Hospital of Anhui Province, Hefei, China

9. Department of Laboratory Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China

10. The Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China

Abstract

While circulating cell-free DNA (cfDNA) has been becoming a powerful marker for noninvasive identification of infectious pathogens in liquid biopsy specimens, a baseline for microbial cfDNA in healthy individuals is urgently needed for the proper interpretation of microbial cfDNA sequencing results in clinical metagenomics. Standard low-pass whole-genome-sequencing-based NIPT shares many similarities with the sequencing protocol for metagenomics and could provide a microbial cfDNA baseline in healthy people; thus, a reference cfDNA data set of the human microbiome was established with sequencing data from a total of 107,763 peripheral blood samples of healthy pregnant women undergoing NIPT screening.

Funder

National Key Research and Development Program of China

CAMS Innovation Fund for Medical Sciences

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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