Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis

Author:

Li Xin-meng12,Shi Xiao3,Yao Yao4,Shen Yi-cun12,Wu Xiang-ling12,Cai Ting12,Liang Lun-xi125ORCID,Wang Fen12ORCID

Affiliation:

1. Department of Gastroenterology, the Third Xiangya Hospital, Central South University, Changsha, Hunan, China

2. Hunan Key Laboratory of Non-resolving Inflammation and Cancer, Central South University, Changsha, Hunan, China

3. Department of Dermatology, Anhui Provincial Hospital, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, China

4. Department of Gastroenterology, Zhangjiajie People’s Hospital, Zhangjiajie, Hunan, China

5. Department of Gastroenterology, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, China

Abstract

The study of gut bacteria has become increasingly popular, and fecal sample preservation methods and times need to be standardized. Here, we detail a 12-month study of fecal sample preservation, and our study provides an empirical reference about experimental details for long-term high-quality storage of fecal samples in the field of gut microbiology research.

Funder

National Natural Science Foundation for Youths of China

Natural Science Foundation of Hunan Province

Scientific research project of Hunan Provincial Health Commission

Hunan Provincial Natural Science Foundation of China for Youths

National Natural Science Foundation of China

Wisdom Accumulation and Talent Cultivation Project of Third Xiangya Hospital of Central South University

Science and Technology Project of Changsha

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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