Comprehensive Assessment of 16S rRNA Gene Amplicon Sequencing for Microbiome Profiling across Multiple Habitats-Reference-Cited by-同舟云学术

Comprehensive Assessment of 16S rRNA Gene Amplicon Sequencing for Microbiome Profiling across Multiple Habitats

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Zhang Wenke¹,Fan Xiaoqian²,Shi Haobo¹,Li Jian¹,Zhang Mingqian¹,Zhao Jin¹,Su Xiaoquan¹^ORCID

Affiliation:

1. College of Computer Science and Technology, Qingdao University, Qingdao, China

2. Shouguang Hospital of Traditional Chinese Medicine, Weifang, China

Abstract

16S rRNA has long been used as a biomarker to identify distinct microbes from complex communities. However, due to the influence of the amplification region, sequencing type, sequence processing, and reference database, the accuracy of 16S rRNA has not been fully verified on a global range.

Funder

Department of Education of Shandong Province

MOST | National Key Research and Development Program of China

National Natural Science Foundation of China

Taishan Scholar Project of Shandong Province

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00563-23

Reference52 articles.