Putative Integrative Mobile Elements That Exploit the Xer Recombination Machinery Carrying bla IMI -Type Carbapenemase Genes in Enterobacter cloacae Complex Isolates in Singapore-Reference-Cited by-同舟云学术

Putative Integrative Mobile Elements That Exploit the Xer Recombination Machinery Carrying bla IMI -Type Carbapenemase Genes in Enterobacter cloacae Complex Isolates in Singapore

Published:2018-01 Issue:1 Volume:62 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Koh Tse H.¹,Rahman Nurdyana Binte Abdul¹,Teo Jeanette W. P.²^ORCID,La My-Van³,Periaswamy Balamurugan⁴,Chen Swaine L.⁴⁵^ORCID

Affiliation:

1. Department of Microbiology, Singapore General Hospital, Singapore

2. Department of Laboratory Medicine, National University Hospital, Singapore

3. National Public Health Laboratory, Singapore

4. Department of Medicine, National University of Singapore, Singapore

5. Infectious Diseases Group, Genome Institute of Singapore, Singapore

Abstract

ABSTRACT Whole-genome sequencing was performed on 16 isolates of the carbapenemase-producing Enterobacter cloacae complex to determine the flanking regions of bla IMI -type genes. Phylogenetic analysis of multilocus sequence typing (MLST) targets separated the isolates into 4 clusters. The bla IMI -type genes were all found on Xer-dependent integrative mobile elements (IMEX). The IMEX elements of 5 isolates were similar to those described in Canada, while the remainder were novel. Five isolates had IMEX elements lacking a resolvase and recombinase.

Funder

MOH | National Medical Research Council

Genome Institute of Singapore

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01542-17

Reference10 articles.

1. Characterization of IMI-1 beta-lactamase, a class A carbapenem-hydrolyzing enzyme from Enterobacter cloacae

2. Characterization of a Novel Putative Xer-Dependent Integrative Mobile Element Carrying theblaNMC-ACarbapenemase Gene, Inserted into the Chromosome of Members of the Enterobacter cloacae Complex