Illuminating the Live-Cell Dynamics of Hepatitis B Virus Covalently Closed Circular DNA Using the CRISPR-Tag System

Author:

Ding Jiahui1,Yi Zhigang1ORCID,Zai Wenjing1,Wu Min2,Chen Baohui3ORCID,Cai Qiliang1ORCID,Zhang Xiaonan24,Yuan Zhenghong12ORCID

Affiliation:

1. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China

2. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China

3. Department of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China

4. Centre for Research in Therapeutic Solutions, Biomedical Sciences, Faculty of Science and Technology, University of Canberra, ACT, Australia

Abstract

Understanding the formation and maintenance of HBV cccDNA has always been a central issue in the study of HBV pathobiology. However, little progress has been made due to the lack of robust assay systems and its resistance to genetic modification.

Funder

National Natural Science Foundation of China

Shanghai Municipal Education Commission

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

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