High-Throughput Neutralization and Serology Assays Reveal Correlated but Highly Variable Humoral Immune Responses in a Large Population of Individuals Infected with SARS-CoV-2 in the US between March and August 2020-Reference-Cited by-同舟云学术

High-Throughput Neutralization and Serology Assays Reveal Correlated but Highly Variable Humoral Immune Responses in a Large Population of Individuals Infected with SARS-CoV-2 in the US between March and August 2020

Published:2023-04-25 Issue:2 Volume:14 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Zhang Shuting¹²³,Ma Peijun¹²³,Orzechowski Marek¹,Lemmer Allison¹,Rzasa Kara¹,Bagnall Josephine¹,Barkho Sulyman¹,Chen Michael¹,He Lorri¹,Neitupski Raymond¹,Tran Victoria¹,Ackerman Ross¹,Gath Emily¹,Bond Austin¹,Frongillo Giana¹,Cleland Thomas¹,Golas Aaron¹,Gaca Anthony¹,Fitzgerald Michael¹,Kelly Kathleen⁴,Hazegh Kelsey⁴,Dumont Larry⁴⁵,Hoffman Corey⁶,Homer Mary⁶^ORCID,Marks Peter⁷,Woolley Ann¹³⁸,Wong Sharon¹,Gomez James¹,Livny Jonathan¹,Hung Deborah¹²³⁸^ORCID

Affiliation:

1. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA

2. Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA

3. Harvard Medical School, Boston, Massachusetts, USA

4. Vitalant Research Institute, Denver, Colorado, USA

5. University of Colorado School of Medicine, Aurora, Colorado, USA

6. Biomedical Advanced Research and Development Authority, Administration for Strategic Preparedness and Response, Department of Health and Human Services, Washington, DC, USA

7. Center for Biologics Evaluation and Research, US FDA, Silver Spring, Maryland, USA

8. Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA

Abstract

The ability to directly measure neutralizing antibodies on live SARS-CoV-2 virus in individuals can play an important role in understanding the efficacy of therapeutic interventions or vaccines. In contrast to functional neutralization assays, serological assays only quantify the presence of antibodies as a proxy of immune protection.

Funder

HHS | Biomedical Advanced Research and Development Authority

Skoll Foundation

U.S. Department of Health and Human Services

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.03523-22

Reference46 articles.

1. Developing antibody tests for SARS-CoV-2