Diagnosis of genitourinary Chlamydia trachomatis infections by using the ligase chain reaction on patient-obtained vaginal swabs

Author:

Hook E W1,Smith K1,Mullen C1,Stephens J1,Rinehardt L1,Pate M S1,Lee H H1

Affiliation:

1. University of Alabama at Birmingham and Jefferson County Department of Health, 35294-0006, USA. ehook@uabid.dom.uab.edu

Abstract

We compared the ligase chain reaction (LCR) assay to cell culture for diagnosis of genitourinary chlamydial infections in women using swab specimens obtained by clinicians from the endocervix and by patients from their own vaginas. Specimens from 40 (12.9%) of 309 patients were positive for chlamydial infection by culture, while the specimens of 50 (16.2%) patients were positive by LCR. Chlamydia trachomatis infection was verified for 9 of 10 patients whose LCR specimens were positive but whose cultures were negative. Vaginal and cervical swab specimens were positive by LCR for 46 (93.9%) and 44 (89.8%) of 49 chlamydia-infected patients, respectively. These data suggest that LCR testing for chlamydia with vaginal swab specimens obtained by patients themselves is as sensitive as cervical LCR and more sensitive than cell culture.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference10 articles.

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2. Diagnosis of Chlamydia trachomatis urethritis in men by polymerase chain reaction assay of first-catch urine;Bauwens J. E.;J. Clin. Microbiol.,1993

3. Centers for Disease Control and Prevention. 1993. Recommendations for the prevention and management of Chlamydia trachomatis infections 1993. Morbid. Mortal. Weekly Rep. 42(No. RR-12):1-39.

4. Division of STD Prevention. Centers for Disease Control and Prevention. 1995. Sexually transmitted disease surveillance 1994. Public Health Service U.S. Department of Health and Human Services Atlanta Ga.

5. Diagnosis of Neisseria gonorrhoeae infections in women by using the ligase chain reaction on patient-obtained vaginal swabs;Hook E. W.;J. Clin Microbiol.,1997

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