Autecological Study of the Chemoautotroph Nitrobacter by Immunofluorescence-Reference-Cited by-同舟云学术

Autecological Study of the Chemoautotroph Nitrobacter by Immunofluorescence

Published:1974-01 Issue:1 Volume:27 Page:124-129
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Fliermans C. B.¹²,Bohlool B. B.¹²,Schmidt E. L.¹²

Affiliation:

1. Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455

2. Department of Soil Science, University of Minnesota, Minneapolis, Minnesota 55455

Abstract

Fluorescent antibodies (FA) prepared for Nitrobacter agilis and N. winogradskyi were highly reactive in homologous staining. Low-level cross-reactions between the two species were removed by adsorption. All 15 pure-culture isolates of Nitrobacter tested reacted strongly with either N. agilis FA or N. winogradskyi FA. All pure-culture isolates from soils were determined to be N. winogradskyi ; those from Mammoth Cave sediments and a cattle waste oxidation ditch were N. agilis . No cross-reaction was found in extensive tests that included five isolates of Nitrosomonas europaea and 668 heterotrophic aerobic and anaerobic bacteria isolated from soil, sewage, and cave sites. The FA preparations were used to detect Nitrobacter species in Mammoth Cave sediments, in a cattle waste oxidation ditch, and in surface waters and sediments of a river and to observe that N. winogradskyi can outgrow N. agilis in enrichment culture.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.27.1.124-129.1974

Reference11 articles.

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