BASP1 Is a Transcriptional Cosuppressor for the Wilms' Tumor Suppressor Protein WT1-Reference-Cited by-同舟云学术

BASP1 Is a Transcriptional Cosuppressor for the Wilms' Tumor Suppressor Protein WT1

Published:2004-01-15 Issue:2 Volume:24 Page:537-549
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Carpenter Brian¹,Hill Kathryn J.¹,Charalambous Marika²,Wagner Kate J.¹,Lahiri Diya²,James Dominic I.¹,Andersen Jens S.³,Schumacher Valérie⁴,Royer-Pokora Brigitte⁴,Mann Matthias³,Ward Andrew²,Roberts Stefan G. E.¹

Affiliation:

1. School of Biological Sciences, University of Manchester, Manchester M13 9PT

2. Developmental Biology Program and Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom

3. Protein Interaction Laboratory, University of Southern Denmark-Odense, DK-5230 Odense, Denmark

4. Institute of Human Genetics and Anthropology, University of Duesseldorf, D40001 Düsseldorf, Germany

Abstract

ABSTRACT The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation by a suppression region within the N terminus of WT1. Using a functional assay, we provide direct evidence that this requires a transcriptional cosuppressor, which we identify as brain acid soluble protein 1 (BASP1). WT1 and BASP1 associate within the nuclei of cells that naturally express both proteins. BASP1 can confer WT1 cosuppressor activity in transfection assays, and elimination of endogenous BASP1 expression augments transcriptional activation by WT1. BASP1 is present in the developing nephron structures of the embryonic kidney and, coincident with that of WT1, its expression is restricted to the highly specialized podocyte cells of the adult kidney. Taken together, our results show that BASP1 is a WT1-associated factor that can regulate WT1 transcriptional activity.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.24.2.537-549.2004

Reference40 articles.

1. Armstrong, J. F., K. Pritchard-Jones, W. A. Bickmore, N. D. Hastie, and J. B. L. Bard. 1992. The expression of the Wilms' tumour gene, WT1, in the developing mammalian embryo. Mech. Dev. 40 : 85-97.

2. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl. 2002. Short protocols in molecular biology 5th ed. vol. 1 p. 16. John Wiley and Sons Inc. New York N.Y.

3. Brummelkamp, T. R., R. Bernards, and R. Agami. 2002. A system for stable expression of short interfering RNAs in mammalian cells. Science 296 : 550-553.

4. Davies, R., A. Moore, A. Schedl, E. Bratt, K. Miyagawa, M. Ladomery, C. Miles, A. Menke, V. van Heyningen, and N. Hastie. 1999. Multiple roles for the Wilms' tumor suppressor, WT1. Cancer Res. 59 : 1747S-1750S.

5. Drummond, I. A., S. L. Madden, P. Rohwernutter, G. I. Bell, V. P. Sukhatme, and F. J. Rauscher. 1992. Repression of the insulin-like growth factor-II gene by the Wilms-tumor suppressor WT1. Science 257 : 674-678.

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