PCR Methods for Rapid Identification and Characterization of Actinobacillus seminis Strains-Reference-Cited by-同舟云学术

PCR Methods for Rapid Identification and Characterization of Actinobacillus seminis Strains

Published:1998-03 Issue:3 Volume:36 Page:814-817
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Appuhamy S.¹,Coote J. G.¹,Low J. C.²,Parton R.¹

Affiliation:

1. Division of Infection and Immunity, University of Glasgow, Glasgow, G12 8QQ,1 and

2. Scottish Agricultural College Veterinary Services, Bush Estate, Penicuik EH26 0QE,2 Scotland, United Kingdom

Abstract

ABSTRACT Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced the simplest pattern and could be useful for identification of A. seminis and for its differentiation from related species. REP- and ERIC-PCR could be used for strain differentiation in epidemiological studies of A. seminis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.3.814-817.1998

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