Author:
Tattersall P,Cawte P J,Shatkin A J,Ward D C
Abstract
Purified full and empty virions of minute virus of mice were separated on CsCl gradients, and their polypeptides were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The empty particle contains two polypeptides, A (83,300 daltons) and B (64,300 daltons), which are 15 to 18% and 82 to 85%, respectively, of the virion mass. The full particle contains the single-stranded DNA genome, proteins A and B, and a third polypeptide, C (61,400 daltons). Again A is 15 to 18% of the protein mass, but the amounts of B and C vary inversely in different preparations of full particles. These polypeptides comprise greater than 99.6% of the protein in either virion, and their molecular weights and molar ratios are independent of the species of host cell on which the virus is propagated, They are not found in uninfected cells, and no protein component of uninfected cells copurifies with either virion under our conditions. Pulse-chase experiments show that the three proteins are synthesized only after virus infection and are therefore probably virus coded. Sequential harvesting from the nuclei of cells infected under one cycle growth conditions shows an increase in the proportion of C in full particles as infection progresses, suggesting that C is derived from B in a late maturation step.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference42 articles.
1. Electron microscope study of human NB and SMH cells infected with the parvovirus, H-1: involvement of the nucleolus;Al-Lami F.;J. Gen. Virol.,1969
2. Processing of adenovirus 2-induced proteins;Anderson C. W.;J. Virol.,1973
3. Transformation of a rat epithelial-like cell line by murine sarcoma virus;Bomford R.;J. Natl. Cancer Inst.,1972
4. Fluorographic detection of 3H, 14C and 35S in acrylamide gels;Bonner W. M.;Eur. J. Biochem.,1974
5. DNA of minute virus of mice: self-priming, nonpermuted, single-stranded genome with a 5'-terminal hairpin duplex;Bourguignon G. J.;J. Virol.,1976
Cited by
164 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献