Association of Murine Leukemia Virus Pol with Virions, Independent of Gag-Pol Expression-Reference-Cited by-同舟云学术

Association of Murine Leukemia Virus Pol with Virions, Independent of Gag-Pol Expression

Published:1999-11 Issue:11 Volume:73 Page:9632-9637
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Buchschacher Gary L.¹,Yu Lei²,Murai Fukashi²,Friedmann Theodore²,Miyanohara Atsushi²

Affiliation:

1. Division of Hematology/Oncology, Department of Medicine,1 and

2. Department of Pediatrics,2 Center for Molecular Genetics, University of California—San Diego, La Jolla, California 92093-0634

Abstract

ABSTRACT During the replication cycle of murine leukemia virus (MLV), Pol is normally synthesized as part of a Gag-Pol fusion protein. In this study, the ability of free MLV Pol to be incorporated into virions was examined. When MLV Gag and MLV Pol were coexpressed from separate plasmids in cells, reverse transcriptase (RT) activity associated with Gag core particles at a slightly lower level than did RT activity generated from wild-type Gag-Pol expression. Particles produced in this manner were somewhat less infectious than those produced with wild-type Gag-Pol. A smaller amount of MLV Pol also associated with heterologous human immunodeficiency virus type 1 Gag cores.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.73.11.9632-9637.1999

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