Author:
Sonenshein A L,Alexander H B,Rothstein D M,Fisher S H
Abstract
Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of sigma-containing molecules of RNA polymerase. Transcription by core enzyme was relatively resistant to the drug, but addition of sigma led to highly drug-sensitive RNA synthesis. We isolated lipiarmycin-resistant mutants of Bacillus subtilis and characterized them genetically and biochemically. Drug-resistant mutants contained an altered RNA polymerase that was resistant to the drug in vitro. By separation and mixed reconstitution of core and sigma fractions of mutant and wild-type RNA polymerase, we showed that lipiarmycin resistance in one mutant strain was a property of the core fraction. Genetic mapping experiments indicated that at least two lpm mutants are located between loci determining rifampin resistance and streptolydigin resistance.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference23 articles.
1. New RNA polymerase from Bacillus subtilis infected with phage PBS2;Clark S.;Nature (London),1974
2. Lipiarmycin, a new antibiotic formActinoplanes. II. Isolation, chemical, biological and biochemical characterization;Coronelli C.;J. Antibiot.,1975
3. Fisher S. D. Rothstein and A. L. Sonenshein. 1975. Ribonucleic acid synthesis in permeabilized mutant and wild-type cells ofBacillus subtilis p. 226-230. In P. Gerhardt R. N. Costilow and H. L. Sadoff (ed.) Spores VI. American Society for Microbiology Washington D.C.
4. New phage-SPOl-induced polypeptides associated with Bacillus subtilis RNA polymerase;Fox T. D.;Proc. Natl. Acad. Sci. U.S.A.,1974
5. Systematic nomenclature for the RNA polymerase genes of prokaryotes;Hayward R. S.;Nature (London),1976
Cited by
52 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献