CHARACTERISTICS OF BRUCELLAPHAGE

Abstract

McDuff , C. R. (University of Wisconsin, Madison), Lois M. Jones, and J. B. Wilson . Characteristics of brucellaphage. J. Bacteriol. 83: 324–329. 1962.—Methods of characterizing phage have been applied to a brucellaphage of Russian origin grown on its propagating strain, Brucella abortus R 19. Phage can be propagated by single plaque transfer. Phage titers of about 10 10 particles per ml can be obtained by propagation on a young culture of R 19 in Albimi broth on a shaker at 37 C. After lyophilization, phage retains its activity during storage for at least 20 months at 4 C. Phage is stable in broth at pH values from 6 to 8 for 24 hr at 37 C. Some loss in activity results from heating for 1 hr at 60 C. All activity is lost in the presence of 10% chloroform. It has a slow adsorption rate (K = 3.6 × 10 −11 ml/min), a latent period of 100 min, and a burst size of 121 particles. Electron micrographs indicate that the phage is approximately 65 mμ in diameter, polygonal in shape, with a short tail.