Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters-Reference-Cited by-同舟云学术

Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters

Published:2005-02 Issue:2 Volume:71 Page:1084-1088
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Miyoshi Tatsuo¹,Iwatsuki Teruki²,Naganuma Takeshi¹²

Affiliation:

1. Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima

2. Tono Geoscience Center, Japan Nuclear Cycle Development Institute, Izumicho, Toki, Japan

Abstract

ABSTRACT A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-μm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-μm-pore-size filters were affiliated with the candidate divisions OD1 and OP11, representing novel lineages. On the other hand, OTUs captured by 0.2-μm-pore-size filters were largely affiliated with Betaproteobacteria .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.2.1084-1088.2005

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