Author:
Barbeyron T,Kean K,Forterre P
Abstract
We have examined the presence of methylated adenine at GATC sequences (Dam phenotype) in the DNA of 23 eubacteria and 13 archaebacteria by using isoshizomer restriction enzymes. We have found a completely Dam+ phenotype in bacteria of nine genera related to the families Enterobacteriaceae, Parvobacteriaceae, and Vibrionaceae, and in the five cyanobacteria tested. We have found a partial Dam+ phenotype in the two archaebacteria Halobacterium saccharovorum and Methanobacterium sp. strain Ivanov. All of the other archaebacteria (three genera) and eubacteria (nine genera) tested were Dam-. Phylogenetic analysis, based on the evolutionary tree of Fox et al. (Science 209:457-463, 1980), indicates that dam methylation in the Escherichia coli lineage appeared recently in bacterial evolution and is restricted to a small range of closely related bacteria.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference33 articles.
1. Evolutionary relationships in Vibrio and Photobacterium: a basis for a natural classification. Annu;Baumann P.;Rev. Microbiol.,1983
2. Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature;Brock T. D.;Arch. Microbiol.,1972
3. The isolation and characterisation of the E. coli adenine methylase dam gene;Broocks J. E.;Nucleic Acids Res.,1983
4. Buchanan R. E. and N. E. Gibbons. 1974. Bergey's manual of determinative bacteriology 8th ed. The Williams & Wilkins Co. Baltimore.
5. Excision and repair of mismatched base pairs in transformation of Streptococcus pneumoniae;Claverys J. P.;Mol. Gen. Genet.,1980
Cited by
97 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献