Affiliation:
1. Biological Sciences Department, Corporate Research Laboratories, Monsanto Company, St. Louis, Missouri 63198
Abstract
Serratia marcescens
, a chitinase-producing microorganism, was shown to produce five unique chitinolytic proteins with subunit molecular masses of 21, 36, 48, 52, and 57 kilodaltons. A cosmid library of
S. marcescens
DNA was constructed in the broad-host-range cosmid pLAFR1 and screened in
Escherichia coli
for clones capable of degrading chitin. A total of four independent clones (22- to 27-kilobase inserts) were isolated, characterized by restriction endonuclease digestion, and shown to share a common 9.5-kilobase
Eco
R1 fragment apparently encoding the same 57-kilodalton chitinase, the most abundant chitinase produced by
S. marcescens
. Chitinase expression from these constructs in both
E. coli
and
Pseudomonas fluorescens
701E1 is apparently driven by an
S. marcescens
promoter. The significantly higher chitinase levels produced in
E. coli
relative to those in
P. fluorescens
701E1 suggest that
E. coli
may recognize this promoter sequence more efficiently than
P. fluorescens.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
139 articles.
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