Comparison of Restriction Fragment Length Polymorphism, Microsatellite Length Polymorphism, and Random Amplification of Polymorphic DNA Analyses for Fingerprinting Aspergillus fumigatus Isolates

Author:

Bart-Delabesse Emmanuelle1,Sarfati Jacqueline2,Debeaupuis Jean-Paul2,van Leeuwen Willem3,van Belkum Alex3,Bretagne Stephane1,Latge Jean-Paul2

Affiliation:

1. Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, Créteil,1 and

2. Unitédes Aspergillus, Institut Pasteur, Paris,2France, and

3. Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands3

Abstract

ABSTRACT Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast to RAPD, RFLP and MLP gave discriminating and significantly concordant genotyping results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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