Affiliation:
1. Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan
Abstract
ABSTRACT
Cladophialophora carrionii
is one of the relatively common causative agents of chromoblastomycosis. We have developed the specific oligonucleotide primer set based on the internal transcribed spacer regions of ribosomal DNA for the rapid identification of this pathogen. PCR with this primer set amplified a 362-bp amplicon from
C. carrionii
strains. From other relevant dematiaceous species, including medically important dematiaceous fungi, such as
Fonsecaea pedrosoi
,
Phialophora verrucosa
, and
Exophiala dermatitidis
, and eight species of medically important yeasts, such as
Candida albicans
and
Cryptococcus neoformans
var.
neoformans
, the primer set did not produce any amplicon. PCR with this primer set may be a useful tool for the identification of
C. carrionii
.
Publisher
American Society for Microbiology
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